文献类型：期刊文献

中文题名：48株爱德华氏菌糖酵解持家酶的胞外分泌

英文题名：Extracellular secretion of glycolytic housekeeping enzymes in 48 strains of Edwardsiella

作者：周翔宇[1];吴海珍[1]

机构：[1]生物反应器工程国家重点实验室应用生物学系华东理工大学

年份：2019

卷号：46

期号：6

起止页码：1282

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金(31872608,31372550)~~

语种：中文

中文关键词：爱德华氏菌;糖酵解持家酶;胞外分泌;ELISA

外文关键词：Edwardsiella;Glycolytic housekeeping enzyme;Extracellular secretion;ELISA

摘要：【背景】病原菌的糖酵解持家酶能分泌到胞外或定位在细胞膜表面,在病原菌的侵染和细胞粘附方面发挥着重要作用,爱德华氏菌是重要的鱼类致病菌,研究其糖酵解持家酶的胞外分泌有助于该病原的致病机制研究和疫苗开发。【目的】探究爱德华氏菌中糖酵解持家酶的胞外分泌。【方法】通过ELISA方法考察48种不同来源的爱德华氏菌中5种糖酵持家酶的胞外分泌。【结果】48种不同来源的爱德华氏菌中糖酵解持家酶蛋白均能分泌到胞外。【结论】爱德华氏菌中糖酵解持家酶的胞外分泌是普遍现象。
[Background] The glycolytic housekeeping enzyme can be secreted to the extracellular matrix or located on the surface of the cell membrane, which plays an important role in the infection and cell adhesion of pathogen. The study of the secretion of glycolytic housekeeping enzymes in Edwardsiella, which is an important fish pathogen, is benefit to its pathogenesis and vaccine development.[Objective] To explore the secretion of housekeeping enzymes in glycolysis of Edwardsiella.[Methods] With ELISA, five housekeeping enzymes in the extracellular proteins from 48 different strains of of Edwardsiella was investigated.[Results] Five glycolytic enzymes are widespread in the extracellular protein of 48 strains of Edwardsiella.[Conclusion] Extracellular secretion of glycolytic enzymes in Edwardsiella is a common phenomenon.