文献类型：期刊文献

英文题名：Functional genes (dsr) approach reveals similar sulphidogenic prokaryotes diversity but different structure in saline waters from corroding high temperature petroleum reservoirs

作者：Guan, Jing[1,2];Zhang, Bing-Liang[1,2];Mbadinga, Serge Maurice[1,2];Liu, Jin-Feng[1,2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China

年份：2014

卷号：98

期号：4

起止页码：1871

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20140917397383);WOS:【SCI-EXPANDED(收录号:WOS:000332106000037)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the NSFC/RGC Joint Research Fund (No. 41161160560).

语种：英文

外文关键词：Corrosion; dsr genes; High sulfate content; High temperature oil reservoir; Saline production water; Sulphidogenic prokaryotes

摘要：Oil reservoirs and production facilities are generally contaminated with H2S resulting from the activity of sulphidogenic prokaryotes (SRP). Sulphidogenesis plays a major role in reservoir souring and microbial influenced corrosion in oil production systems. In the present study, sulphidogenic microbial diversity and composition in saline production fluids retrieved from three blocks of corroding high temperature (79 similar to 95 degrees C) oil reservoirs with high sulfate concentrations were investigated by phylogenetic analyses of gene fragments of the dissimilatory sulfite reductase (dsr). Analysis of dsr gene fragments revealed the presence of several clusters of sulphidogenic prokaryotes that cover the orders Desulfovibrionales (Desulfovibrio, Desulfomicrobium thermophilum), Desulfobacterales (Desulfobacterium, Desulfosarcina, Desulfococcus, Desulfotignum, Desulfobotulus, Desulfobulbus), Syntrophobacterales (Desulfacinum, Thermodesulforhabdus, Desulforhabdus), Clostridiales (Desulfotomaculum) and Archaeoglobales (Archaeoglobus); among which sequences affiliated to members of Desulfomicrobium, Desulfotomaculum and Desulfovibrio appeared to be the most encountered genera within the three blocks. Collectively, phylogenetic and non-metric multidimensional scaling analyses indicated similar but structurally different sulphidogenic prokaryotes communities within the waters retrieved from the three Blocks. This study show the diversity and composition of sulphidogenic prokaryotes that may play a role in the souring mediated corrosion of the oilfield and also provides a fundamental basis for further investigation to control oil reservoir souring and corrosion of pipelines and topside installations.