文献类型：期刊文献

英文题名：A pH-responsive hybrid fluorescent nanoprober for real time cell labeling and endocytosis tracking

作者：Chang, Shu[1];Wu, Xumeng[2,3];Li, Yongsheng[1];Niu, Dechao[1];Gao, Yongping[1];Ma, Zhi[4];Gu, Jinlou[1];Zhao, Wenru[1];Zhu, Weihong[2,3];Tian, He[2,3];Shi, Jianlin[1,5]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Lab Low Dimens Mat Chem,Minist Educ, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China;[5]Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China

年份：2013

卷号：34

期号：38

起止页码：10182

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20134216861662);WOS:【SCI-EXPANDED(收录号:WOS:000328094600043)】；

基金：This work was financially supported by National 973 Program (2012CB933602, 2013CB733700), National Natural Science Foundation of China (51172070, 51132009, 51202068 and 61077085), the Oriental Scholarship, SRFDP 20120074110002, Program for New Century Excellent Talents in University (NCET-10-0379), Shuguang Project (11SG30), Scientific Innovation Project of Shanghai Municipal Education Commission (11ZZ53), Fundamental Research Funds for the Central Universities (222201313010, WD1114002, WD1124010 and WK1013002).

语种：英文

外文关键词：Copolymer; Self assembly; Nanoparticle; Fluorescence; Biosensor; Image analysis

摘要：Hydrophilic, fluorescent hybrid nanoprobes (NDI@HNPs) encapsulated with the hydrophobic pH-responsive fluorophore (N,N'-di-n-dodecy1-2,6-di(4-methyl-piperazin-1-yl)naphthalene-1,4,5,8-tetracarboxylic acid diimide, NDI) for recognizing and mapping the route of cell phagocytosis have been fabricated based on the self-assembly of amphiphilic diblock copolymer PS-b-PAA and the subsequent shell cross-linking with 3-mercaptopropyltrimethoxy silane (MPTMS). The as-synthesized NDI@HNPs has a typical spherical morphology of 46 nm in diameter with excellent monodispersity in aqueous solution. The NDI@HNPs probe exhibits extremely low cytotoxicity, fast real time pH response and enhanced fluorescence intensity under acidic environment with respect to the corresponding free dye in highly polar aqueous system because of the encapsulation of NDI molecules inside nanoparticle cores with weak polarity environment. The fluorescence intensity of NDI@HNPs is enhanced by 55-fold upon changing from neutral (pH = 7.4) or basic (pH = 8.4) to acid (pH = 3.4) in aqueous system, in contrast to the serious fluorescence quenching of free NDI in the same medium, which can exactly meet the physiological pH range in cells. The favorably long emission wavelength is beneficial to the low scattering and minimal interfering requirements to fluorescent bioimaging. Moreover, functionalization with rapid cell-penetrating peptides (HIV-1 TAT) allows them to overcome the physiological and biological barriers during the phagocytosis process. Its characteristic fluorescent response to pH benefits the intracellular labeling and organelle targeting, realizing the real time tracking of the probe entry into cancer cells, the accumulation into the endolysosome and the further escape. (C) 2013 Elsevier Ltd. All rights reserved.