文献类型：期刊文献

英文题名：Highly Selective Detection of Carbon Monoxide in Living Cells by Palladacycle Carbonylation-Based Surface Enhanced Raman Spectroscopy Nanosensors

作者：Cao, Yue;Li, Da-Wei[1];Zhao, Li-Jun;Liu, Xiao-Yuan;Cao, Xiao-Ming;Long, Yi-Tao

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：87

期号：19

起止页码：9696

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20154001344304);WOS:【SCI-EXPANDED(收录号:WOS:000362628600030)】；

基金：This research was supported by the National Basic Research 973 Program (Grant 2013CB733700), the NSFC (Grants 21421004, 21125522, 21327807, 21575041), the Shanghai Pujiang Program Grant of China (Grant 12JC1403500), the Shanghai Municipal Natural Science Foundation (Grant 14ZR1410800), and the Fundamental Research Funds for the Central Universities (Grant WB1113005).

语种：英文

外文关键词：Fiber optic sensors - Raman spectroscopy - Synthesis (chemical) - Nanosensors - Lanthanum compounds - Gold nanoparticles - Light transmission - Carbonylation

摘要：A novel nanosensor was explored for the highly selective detection of intracellular carbon monoxide (CO) by surface enhanced Raman spectroscopy (SERS) on the basis of palladacyde carbonylation. By assembling new synthesized palladacydes (PC) on the surface of gold nanoparticles (AuNPs), SERS nanosensors (AuNP/PC) were prepared with good SERS activity and reactivity with CO. When the AuNP/PC nanosensors were incubated with a CO-containing system, carbonylation of the PC assembled on AuNPs was initiated, and the corresponding SERS spectra of AuNP/PC changed significantly, which allowed the carbonylation reaction to be directly observed in situ. Upon SERS observation of CO-dependent carbonylation, this SERS nanosensor was used for the detection of CO under physiological conditions. Moreover, benefiting from the specificity of the reaction coupled with the fingerprinting feature of SERS, the developed nanosensor demonstrated high selectivity over other biologically relevant species. In vivo studies further indicated that CO in normal human liver cells and HeLa cells at concentrations as low as 0.5 mu M were successfully detected with the proposed SERS strategy, demonstrating its great promise for the analytical requirements in studies of physiopathological events involved with CO.