文献类型：期刊文献

英文题名：Negative Electrets Directed Fibrinogen Assembly for Surface Enrichment of Platelets

作者：Wang, Zhong[1];Liu, Yuji[2];Geng, Hao[3];Qiu, Xunlin[2];Qin, Hui[4];Cao, Huiliang[1,5,6]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Interfacial Electrochem & Biomat, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Mech & Power Engn, Shanghai Key Lab Intelligent Sensing & Detect, Shanghai, Peoples R China;[3]Changzhou Univ, Adv Carbon Mat Res Ctr, Sch Mat Sci & Engn, Changzhou, Peoples R China;[4]Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthoped, Shanghai, Peoples R China;[5]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai, Peoples R China;[6]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai, Peoples R China

年份：2026

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;EI(收录号：20261320354707);WOS:【SCI-EXPANDED(收录号:WOS:001720209900001)】；

基金：This work was jointly supported by grants from the National Natural Science Foundation of China (Nos. 32271399, 31870945, 12474467, and 52321002), the Natural Science Foundation of Shanghai (21ZR1415700), and the Shanghai Committee of Science and Technology (23S31901700).

语种：英文

外文关键词：fibrinogen; implantable medical devices; platelet-rich plasma; protein adsorption; surface charge

摘要：Platelet-encapsulated growth factors are desired for rapid tissue healing and personalized regeneration; however, guided platelet enrichment on implantable biomaterial surfaces still faces challenges, undermining the clinical effects of commercial platelet concentrates on tissue integration. Here, we demonstrated that high intensity of negative charge on fluorinated ethylene propylene electrets (ranging from -1252 +/- 66 V/-0.465 +/- 0.025 mC center dot m-2 to -2838 +/- 95 V/-1.055 +/- 0.035 mC center dot m-2) can direct self-assembly of fibrinogen and guide platelet adhesion for surface enrichment. Detailed fibrinogen adsorption tests with a series of protein concentrations and incubation durations showed that the negative electrets induced fibrinogen networks quickly and were more porous compared to the positive ones. In addition, an extremely low binding energy of X-ray photoelectron spectroscopy N1s (398.2 eV) and Fermi resonance associated Amide A and Amide B bands were exclusively detected on fibrinogen-incubated negative electrets, indicating that the negative surface charge interacted with the fibrinogen via producing and accumulating orient water hydrated sodium ions, which coordinate with the gamma 400-401 histidine dipeptides in the gamma C of the absorbent fibrinogen D domains and facilitated the exposure of the gamma 400-411 sequences, mediating platelet adhesions on the electrets free of premature activation. Since fibrinogen is a naturally present protein in the human body, this proof-of-concept study provides new insights into advancing implantable biomaterials to orchestrate tissue integration at the protein and cellular levels.