文献类型：期刊文献

英文题名：A novel colorimetric and ratiometric fluorescent probe for selective detection of bisulfite in real samples and living cells

作者：Li, Jin[1];Gao, Ying[1];Guo, Haoran[1];Li, Xiaokang[2];Tang, Haoyang[3];Li, Jian[2];Guo, Yuan[1]

机构：[1]Northwest Univ, Coll Chem & Mat Sci, Natl Demonstrat Ctr Expt Chem Educ, Key Lab Synthet & Nat Fratct Mol Chem,Minist Educ, Xian 710127, Shaanxi, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai 20023Z, Peoples R China;[3]Xian Univ Posts & Telecommun, Sch Automat, Xian 710121, Shaanxi, Peoples R China

年份：2019

卷号：163

起止页码：285

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20185006240570);WOS:【SCI-EXPANDED(收录号:WOS:000457666300036)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21472148 and 21072158), Open Funding Project of the State Key Laboratory of Bioreactor Engineering, Academic Backbone of Northwest University Outstanding Youth Support Program, Technology Plan Project of Xi'an [No. 201805040YD18CG24(1)] and the Top-Rated Discipline Construction Scheme of Shaanxi Higher Education.

语种：英文

外文关键词：Colorimetric and ratiometric; Fluorescent probe; Bisulfite; Real samples; Live-cell imaging

摘要：An abnormal level of bisulfite can induce toxicological effects associated with lung cancer, cardiovascular and neurological disorders. Therefore, it is of significance to develop an effective fluorescent probe to detect bisulfite in living cells. Herein, a novel fluorescent probe QPCT, based on a 1,4-addition mechanism, was constructed for the colorimetric and ratiometric detection of bisulfite. QPCT displayed high selectivity and anti-interference ability to bisulfite over other anions and biothiols. The probe renders a sensitive ratiometric response to bisulfite with a remarkable fluorescence blue shift from 590 to 537 nm and the fluorescence ratio was linear with bisulfite concentration over the range of 0-120 mu M. More importantly, QPCT has been successfully applied to detect bisulfite in sugars and living A549 cells, which indicated that QPCT had a great capability for monitoring bisulfite in complex systems.