文献类型：期刊文献

英文题名：Medium optimization for meilingmycin production by Streptomyces nanchangensis using response surface methodology

作者：Zhuang, YP; Chen, B; Chu, J; Zhang, SL

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Natl Engn Res Ctr Biotechnol, Shanghai 200237, Peoples R China

年份：2006

卷号：41

期号：2

起止页码：405

外文期刊名：PROCESS BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000234624200022)】；

语种：英文

外文关键词：meilingmycin; medium; optimization

摘要：Statistically based experimental designs were used to optimize the medium for meilingmycin (MLM) production by Streptomyces nanchangensis M-6. In the first optimization step the effects of corn flour, maize starch, soybean meal, yeast meal, K2HPO4, KNO3, soybean oil, NaCl and CaCO3 on MLM production were evaluated by using a fractional factorial design. The production of MLM was favored by using corn flour or maize starch, but adversely affected by higher level of yeast meal. Other components exerted no significant influence on MLM production. The path of the steepest ascent was used to access the optimal region of the medium composition. In the third step, the concentrations of corn flour and maize starch were further optimized with central composite design and response surface analysis. This optimization strategy led to the enhancement of MLM production from 51.3 to 228.5 mu g/mL. (c) 2005 Elsevier Ltd. All rights reserved.