文献类型：期刊文献

中文题名：代谢工程—生物工程学科的新兴研究领域

英文题名：METABOLIC ENGINEERING: A RISING FIELD IN BIOCHEMICAL ENGINEERING

作者：沈立新[1];魏东芝[1];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,生物化学研究所,上海200237

年份：2000

卷号：20

期号：3

起止页码：47

中文期刊名：微生物学杂志

外文期刊名：Journal of Microbiology

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：代谢工程;推理性代谢工程;逆代谢工程;生物工程

外文关键词：metabolic engineering, constructive metabolic engineering, inverse metabolic engineering, DNA technology, bioactivity.

摘要：代谢工程包括推理性代谢工程及逆代谢工程。针对限制生物活性的因素 ,从不同的途径设计代谢改变策略 ,采用重组DNA技术解除或削弱该影响 ,提高目的产物的产率 ,是生化工程学科提高生物活性的新兴研究领域。讨论了推理性代谢工程及逆代谢工程的设计及应用。
Metabolic engineering includes constructive metabolic engineering and inverse metabolic engineering. Recombinant DNA technology is used to improve cellular activities after the targets of the metabolic modification are determined. The targets are based on analysis of metabolic network for constructive metabolic engineering. Inverse metabolic engineering elucidates the strategy by identifying or calculating a desired phenotype, determining the genetic factors conferring that phenotype. Metabolic engineering and inverse metabolic engineering are new effective and reasonable means to improve strains of biochemical engineering. Processes and application are briefly introduced.\;