文献类型：期刊文献

英文题名：A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate

作者：Hang, Haifeng[1];Ye, Xinhao[1];Guo, Meijin[1,2];Chu, Ju[1];Zhuang, Yingping[1];Zhang, Ming[1];Zhang, Siliang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiangxi Agr Univ, Dept Biotechnol, Nanchang 330045, Peoples R China

年份：2009

卷号：44

期号：4

起止页码：185

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000263759300001)】；

基金：The authors gratefully acknowledge financial support from the Natural Science Foundation Commission of China (No. 30560006), High-Tech Research Plan of China (863) (2007AA100601) and National Basic Research Program (No. 2007CB714303).

语种：英文

外文关键词：Pichia pastoris; Phytase; Glucose; Feeding strategy

摘要：The methylotrophic yeast Pichia pastoris has become a popular host for the production of recombinant protein under the tight control of methanol-induced alcohol oxidase promoter (P-AOX1), which is, however, strongly repressed by glucose at the transcription level. A real-time parameter-based controlled glucose feed strategy (rpcGFS) is developed here to realize Pichia high-cell density culture. It allows for the high-level production of recombinant phytase by P. pastoris YY113 using glucose as the growth substrate instead of glycerol. As a result, high phytase production (2200 FTU phytase ml(-1)) and yield (0.25 FTU ml(-1) [phytase] g(-1)[methanol]) are achieved successfully, respectively, after 100 h methanol induction by this novel simple fermentation strategy. (C) 2008 Elsevier Inc. All rights reserved.