文献类型：期刊文献

英文题名：Natural pyrethrins induces apoptosis in human hepatocyte cells via Bax- and Bcl-2-mediated mitochondrial pathway

作者：Yang, Yun[1];Zong, Mimi[1];Xu, Wenping[1];Zhang, Yang[1];Wang, Bo[1];Yang, Mingjun[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2017

卷号：262

起止页码：38

外文期刊名：CHEMICO-BIOLOGICAL INTERACTIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390665400005)】；

基金：This work was financial supported by National Key Technology Research Development Program of China (NO. 2011BAE06B04).

语种：英文

外文关键词：Natural pyrethrins; Cytotoxic effects; Human hepatocyte cells; Apoptosis; The mitochondrial pathway

摘要：Natural pyrethrins have been widely used for pest control in organic farming and for residential indoor pest managements. Although the specific mechanisms underlying their activity are incompletely understood, natural pesticides are considered the safest based on their target specificity and rapid degradation in the environment. Here, we used in vitro bioassays to characterize the cytotoxic effects of natural pyrethrins and attempted to delineate the cellular and molecular mechanisms of their cytotoxicity against human hepatocytes. The results demonstrate that natural pyrethrins reduce cell viability and enhance apoptosis in HepG2 cells. In addition, the current data indicate that natural pyrethrins cause a reduction in the mitochondrial membrane potential (Delta Psi m), increase reactive oxygen species production, and up-regulate the Bax/Bcl-2 expression, leading to the release of cytochrome-c into the cytosol, activation of caspase-9 and caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP). Taken together, the results indicate that natural pyrethrins has potentially exert adverse effects on human health by inducing caspase-dependent apoptosis in hepatocytes through Bax-and Bcl-2-mediated mitochondrial pathway. (C) 2016 Elsevier Ireland Ltd. All rights reserved.