文献类型：期刊文献

英文题名：A Novel (R)-Imine Reductase from Paenibacillus lactis for Asymmetric Reduction of 3H-Indoles

作者：Li, Hao[1];Zhang, Guang-Xiang[1];Li, Liu-Mei[1];Ou, Yu-Shi[1];Wang, Ming-Yang[1];Li, Chun-Xiu[1];Zheng, Gao-Wei[1];Xu, Jian-He[1]

机构：[1]E China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：8

期号：4

起止页码：724

外文期刊名：CHEMCATCHEM

收录：;EI(收录号：20160902003839);WOS:【SCI-EXPANDED(收录号:WOS:000371222600010)】；

基金：This work was financially supported by the National Natural Science Foundation of China ( No. 31200050, 21472045 and 21536004) and the Fundamental Research Funds for the Central Universities (22A201514043).

语种：英文

外文关键词：asymmetric reduction; biocatalysis; imine reductase; indoles; indolines

摘要：A novel (R)-imine reductase (PlRIR) from Paenibacillus lactis was heterologously overexpressed in Escherichia coli, purified and characterized. The purified PlRIR exhibited relatively high catalytic efficiency (k(cat)/K-m=1.58s(-1)mm(-1)) towards 2,3,3-trimethylindolenine. A panel of 3H-indoles and 3H-indole iodides were reduced by PlRIR to yield the corresponding products with good-to-excellent enantioselectivity (66-98% ee). In addition, PlRIR also possesses good activities toward other types of imines such as pyrroline, tetrahydropyridine, and dihydroisoquinoline, indicating a reasonably broad substrate acceptance. In a 100mg scale preparative reaction, 100mm 2,3,3-trimethylindolenine was converted efficiently to afford (R)-2,3,3-trimethylindoline with 96% ee and 81% yield.