文献类型：期刊文献

中文题名：冷冻保护剂对丹参酮脂质体冻融后粒度分布的影响及机理考察

英文题名：Influence of Cryoprotectants on Particle Size Distribution of Freeze-Thawing Tanshinone Liposomes and Cryoprotective Mechanism

作者：储茂泉[1];古宏晨[2];刘国杰[1]

机构：[1]华东理工大学化学与制药学院;[2]教育部超细材料制备与应用重点实验室,上海200237

年份：2002

卷号：33

期号：9

起止页码：445

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：冷冻保护剂;丹参酮脂质体;冻融;粒度分布;影响;机理

外文关键词：tanshinone liposomes; freeze thawing method; cryoprotectant; particle size

摘要：考察了单糖、二糖、三糖、多醇、二甲亚砜以及高分子 PVP等附加剂对冻融 3次的丹参酮脂质体粒度的影响及机理 ,结果表明 ,所选附加剂均能不同程度地抑制丹参酮脂质体在冻融后粒子的显著增大 ,附加剂对冻融脂质体起保护作用的机理包括脂质体的静电斥力增大和附加剂的空间位阻的协同作用 ,以及附加剂减少了脂质体周围的冰晶等。
The influence of additives, such as mono ,di and tri glucosides, polyalcohols, dimethyl sulphoxide (DMSO) and PVP, on the particle size distribution of tanshinone liposomes freeze thawed three times were investigated. Results showed that additives mentioned above could prevent the particle size of tanshinone liposomes increased significantly after freeze thawing process. The cryoprotective mechanism of these additives on freeze thawing of tanshinone liposomes was attributed to zeta potential of liposomes increased by PVP, the barrier of additives between liposomes, membrane damage reduced by additives during freeze thawing process, and so on.