文献类型：期刊文献

英文题名：Improved synthesis of 2,5-bis(hydroxymethyl)furan from 5-hydroxymethylfurfural using acclimatized whole cells entrapped in calcium alginate

作者：Xu, Zhong-Hua[1];Cheng, Ai-Di[1];Xing, Xu-Pu[1];Zong, Min-Hua[1];Bai, Yun-Peng[2];Li, Ning[1]

机构：[1]South China Univ Technol, Sch Food Sci & Engn, 381 Wushan Rd, Guangzhou 510640, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：262

起止页码：177

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20181805130624);WOS:【SCI-EXPANDED(收录号:WOS:000433219500022)】；

基金：This research was financially supported by the Natural Science Foundation of Guangdong Province, China (2017A030313056), the Fundamental Research Funds for the Central Universities (2017ZD065), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. The authors are grateful to Dr. Xue-Dan Hou of Guangdong University of Technology for providing rice straw hydrolysate.

语种：英文

外文关键词：Biocatalysis; Biobased platform chemicals; Immobilization; Reduction; Whole cells

摘要：Upgrading of biomass-derived 5-hydroxymethylfurfural (HMF) has attracted considerable interest recently. In this work, efficient synthesis of 2,5-bis(hydroxymethyl) furan (BHMF) from HMF was reported with the acclimatized Meyerozyma guilliermondii SC1103 cells entrapped in calcium alginate beads. Catalytic activities of the cells as well as their HMF-tolerant level increased significantly upon acclimatization and immobilization. BHMF was obtained within 7-24 h with good yields (82-85%) and excellent selectivities (99%) when the substrate concentrations were 200-300 mM. In scale-up synthesis, BHMF of up to 181mM was produced within 7 h, and its productivity was approximately 3.3 g/L h. In addition, the immobilized biocatalyst showed satisfactory operational stability; the cell viability of 70% was retained after reuse 4 times. With rice straw hydrolysate as cosubstrate, both the reaction rate and selectivity decreased, likely due to the deleterious influence of xylose in the hydrolysate.