文献类型：期刊文献

英文题名：Integrated microfluidic platforms for tumor-derived exosome analysis

作者：Xu, Huiying[1]; Ye, Bang-Ce[1]

机构：[1] Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2023

卷号：158

外文期刊名：TrAC - Trends in Analytical Chemistry

收录：EI(收录号：20224913218216)

语种：英文

外文关键词：Biomarkers - Body fluids - Diagnosis - Diseases - Phospholipids - RNA - Tumors

摘要：Exosomes are membrane-surrounded phospholipid nanovesicles secreted by most types of cells and circulate in body fluids. Tumor-derived exosome packaged with abundantly tumor-specific proteins, functional messenger RNAs and microRNAs from their original cells, thereby emerging as reliable noninvasive biomarkers in monitoring cancer occurrence and dynamic progression. Conventional techniques to isolate and characterize exosomes usually require sophisticated instruments and complicated multi-step workflows, restricting the development and transformation of exosome-oriented analysis in clinical application. As an alternative, microfluidics offers versatile platforms for integrating efficient isolation and in situ analysis of exosomes on a single chip. In this review, we would focus on the integrated microfluidic platforms for tumor-derived exosomes isolation and downstream analysis, and provide a comprehensive overview of recent progress according to different research perspective from exosomal surface biomarkers to inside cargo molecules, which hold tremendous potential for early cancer screening, prognostic monitoring, and personalized medicine. ? 2022 Elsevier B.V.