文献类型：期刊文献

英文题名：RQ feedback control for simultaneous improvement of GSH yield and GSH content in Saccharomyces cerevisiae T65

作者：Xiong, Zhi-Qiang[1,2]; Guo, Mei-Jin[1]; Guo, Yuan-Xin[1]; Chu, Ju[1]; Zhuang, Ying-Ping[1]; Wang, Nam Sun[2]; Zhang, Si-Liang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, United States

年份：2010

卷号：46

期号：7

起止页码：598

外文期刊名：Enzyme and Microbial Technology

收录：EI(收录号：20102112948925)

语种：英文

外文关键词：Oxygen - Proportional control systems - Two term control systems - Glucose - Peptides - Yeast - Ethanol - Carbon dioxide - Productivity

摘要：In reduced glutathione (GSH) production by high-cell-density fed-batch cultivation of Saccharomyces cerevisiae T65, a respiratory quotient (RQ) feedback control strategy was applied to determine glucose feeding rate (F) based on on-line off-gas monitoring in a 15-l stirred bioreactor. Glucose feed was manipulated by a classical proportional-integral-derivative (PID) controller to control RQ at its set-point. RQ controlled at 0.65 resulted in the highest GSH productivity (46.9. mg/l/h) and cell productivity (3.5. g/l/h) as well as improved GSH yield and intracellular GSH content. RQ feedback control achieved yeast dry weight (126. g/l), GSH yield (2.1. g/l), GSH content (1.67%) and GSH productivity (55.3. mg/l/h), which improved by 11.5%, 75%, 57.5% and 82.5%, respectively, compared with conventional ethanol feedback control in the GSH industry. Moreover, RQ feedback control reduced ethanol (byproduct) level to below 0.3. g/l. Advantages of RQ feedback control over the conventional ethanol feedback control in the GSH industry include lower byproduct concentration, shorter cultivation period, higher GSH content, and higher GSH yield. Hence, feedback control based on RQ is a promising method that poses as a simple and efficient alternative to conventional feed control techniques presently practiced in the GSH industry. ? 2010.