文献类型：期刊文献

英文题名：Overhang molecular beacons encapsulated in tethered cationic lipoplex nanoparticles for detection of single-point mutation in extracellular vesicle-associated RNAs

作者：Hu, Jiaming[1,2];Kwak, Kwang Joo[3];Shi, Junfeng[3];Yu, Bohao[4];Sheng, Yan[1,2];Lee, Ly James[3]

机构：[1]South China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China;[2]South China Normal Univ, Coll Biophoton, Inst Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China;[3]Ohio State Univ, William G Lowrie Dept Chem & Biomol Engn, Columbus, OH 43210 USA;[4]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：183

起止页码：20

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20183805820320);WOS:【SCI-EXPANDED(收录号:WOS:000447116600003)】；

基金：The authors acknowledge the financial supports from National Natural Science Foundation of China [grant number 31741050], National Natural Science Foundation of China [grant number 21804023] and the National Science Foundation, USA [grant number EEC-0914790]. The human serum samples were kindly donated from the First Affiliated Hospital of Bengbu Medical College, China.

语种：英文

外文关键词：Extracellular vesicle; Overhang molecular beacon; Tethered cationic lipoplex nanoparticle; Cancer diagnosis; Single-point mutation

摘要：Detection of specific extracellular RNAs has been developed for non-invasive cancer diagnosis. However, accurate and efficient identification of RNAs with single-point mutation in cancer cells-derived extracellular vesicles (EVs) is challenging. Herein, we present a unique overhang molecular beacon with internal dye (Ohi-MB) with a stable hairpin structure, fast hybridization kinetics and single mismatch specificity. Ohi-MBs are encapsulated in cationic lipoplex nanoparticles (CLNs) that are tethered on a gold coated glass slide as a chip, which can capture circulating EVs and detect encapsulated target RNAs in-situ in a single step. The capability of detection of single-point mutation by CLN-Ohi-MB is demonstrated in artificial EVs and cancer cells. This CLN-Ohi-MB biochip could quantify single-point mutations in KRAS mRNA (G12C, G12D, G12V) in pancreatic cancer cell-derived EVs and single-point mutations in EGFR mRNA (L858R and T790M) in lung cancer cell-derived EVs with high specificity, not achievable by conventional molecular probes. We show that CLN-Ohi-MB biochip could selectively and sensitively identify single-point mutations in KRAS mRNA in human serum EVs, distinguishing pancreatic cancer patients with different mutations.