文献类型：期刊文献

中文题名：产核苷磷酸化酶大肠杆菌的培养及2′-脱氧-5-氟尿苷的酶法合成

英文题名：Culture of Phosphorylase Forming E.coli Cells and Enzymatic Preparation of 2′ Deoxy 5 fluoro uridine

作者：周长林[1];邱蔚然[1];王欣荣[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：1997

卷号：23

期号：5

起止页码：530

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家"九五"攻关基金

语种：中文

中文关键词：核苷磷酸化酶;大肠杆菌;脱氧氟脲苷;酶法

外文关键词：culture;phosphorylase;2' deoxy 5 fluorouridine;biotransformation

摘要：研究了产核苷磷酸化酶大肠杆菌的摇瓶培养和发酵罐培养特征以及乙酸盐和底物诱导对核苷磷酸化酶活力的影响。结果表明：摇瓶培养时，延长菌体培养时间，菌体部分自溶时酶的转化率最高；发酵培养基中加入１０ｇ／Ｌ乙酸钠可使底物转化率提高７．７％，０．００１ｇ／Ｌ的底物ｄＵ诱导可使酶活力提高１１．０％；流加底物ｄＵ对酶反应转化率无显著影响，表明该酶促反应为非２′－脱氧尿苷底物抑制型；反应过程存在扩散控制，酶反应的最佳摇床转速为１６０ｒ／ｍｉｎ。
The culture of nucleoside phosphorylase forming E.coli cells in shaking flask and fermenter(5L) was studied.The effect of acetate and substrate inducing on the phosphorylase activity were also investivated.It was shown that the enzyme activity was highest when E.coli cells began to autolyse in the prolonging course of culture in shaking flask and increased by 7.7% or 11.0%,either by adding 10g/L sodium acetate or 0.001g/L dU inducer in fermentation medium respectively.In addition,there was not substrate dU inhibition in enzyme reaction because the obvious change of conversion rate was not observed in dU fed batch process.Diffusion control was appeared in biotransformation and the optimal shaker speed was 160r/min.