文献类型：期刊文献

英文题名：Influence of Al3+ on the titer of spiramycin and effective components in fermentor

作者：Li, Zhen[1];Hu, Fengxian[1];Ye, Ruifang[1];Lv, Heping[2];Zeng, Jun[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Topfond Pharmaceut Co Ltd, Dept Res & Dev, Zhumadian, Henan, Peoples R China

年份：2017

卷号：47

期号：5

起止页码：481

外文期刊名：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000402012500007)】；

基金：This work was financially supported by Topfond Pharmaceutical Co., Ltd, Henan, China.

语种：英文

外文关键词：Al3+; Streptomyces spiramyceticus 16-10-2; Spiramycin

摘要：Spiramycin is a multicomponent antibiotic, and different components have different antibacterial activities. In Streptomyces spiramyceticus 16-10-2, spiramycin II and spiramycin III (SPMII and SPMIII) are the main components, while spiramycin I (SPMI) needs to be controlled below 12%. Based on this, the influences of Al3+ on total spiramycin titer and components were investigated in this work. Those experiments were mainly performed in 15L fermentor and Al3+ made a great improvement in spiramycin titer. The optimal adding concentration and adding time of Al3+ were 0.32g/L at 12hr. Under this condition, spiramycin titer was increased by 19.51% compared with the control. Moreover, the percentage of SPMII and SPMIII was increased by 7.14%. At the same time, the time of mycelia autolysis was lengthened. In addition, the specific activities of acetyl-CoA synthetase, acetate kinase, acetylphosphotransferase, and acylating enzyme were much higher than those of control. The content of acetic acid and succinic acid was beyond 3 and 4.5 times than that of control, respectively.