文献类型：期刊文献

英文题名：A portable paper-based analytical device mediated by transition metal selenide nanozymes based on Hg2+-activated oxidase-like activity

作者：Wang, Jingkang[1];Shen, Mingping[1];Meng, Fanxing[1];Han, Xin[2];Zhang, Minwei[1]

机构：[1]Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China;[2]East China Univ Sci & Technol, Sch Mech & Power Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：512

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20251618264371);WOS:【SCI-EXPANDED(收录号:WOS:001475904800001)】；

基金：This work was supported by the Science and Technology Young Top-Notch Talent Project of Autonomous Region (2022TSYCCX0064) , Key Technology Research and Development Program in Autonomous Region (2022A02009-3) , and the Autonomous Region universities basic research funds projects-cultivation projects (XJEDU2023P016) .

语种：英文

外文关键词：Transition metal selenide; Hg2+-responsive oxidase-like activity; Dual-mode; Colorimetric; Smartphone-based test strip

摘要：The booming development of nanozymes opens up great prospects for multimodal analysis of Hg contamination scenarios. However, most nanozymes with ideal performance have complex surface modification procedures. The resulting defects, such as the masking of the active site and low binding efficiency to the target, have hindered their application in the Hg2+ detection field. To this end, six transition metal selenide (TMS) nanozymes (CoSe2, NiSe2, MoSe2, WSe2, MnSe, and CuSe) with Hg2+-responsive oxidase-like (OXD-like) activity were successfully synthesized utilizing the efficient biomimetic design of the nanozymes in this study. Furthermore, the Hirshfeld charge and formation energy of the TMS-Hg2+ reaction were investigated using density functional theory (DFT). The calculation and analysis of charge density differences revealed the electron transfer pathways and flow direction during the binding and electron coupling of Hg-Se bonds. Therefore, the mechanism of the enhanced TMS-Hg2+ OXD-like activity (which accelerates electron transport in the system due to the generation of electron transfer channels by the Hg-Se bond) was clarified, and the ideal TMS nanozyme (CoSe2) most sensitive to Hg2+ was determined. On this basis, a dual-mode analysis system for Hg2+ detection by solution colorimetry and smartphone-based portable test strip platform was developed and effectively utilized to assay Hg2+ in lettuce spiked samples, reducing the detection time to 2 min. Therefore, this study provides valuable insights into the efficient biomimetic recognition design of nanozymes and the application of cost-effective and real-time detection in mercury contamination scenarios.