文献类型：期刊文献

英文题名：Decondensation of cationic gemini surfactant-induced DNA aggregates using triblock copolymer (PEO)20-(PPO)70-(PEO)20

作者：He, Yunfei[1]; Xu, Shouhong[1]; Sun, Di[1]; Shang, Yazhuo[1]; Zhao, Xiaofang[1]; Liu, Honglai[1]

机构：[1] Key Laboratory for Advanced Materials, Department of Chemistry, East China University of Science and Technology, Shanghai 200237, China

年份：2013

卷号：291

期号：9

起止页码：2139

外文期刊名：Colloid and Polymer Science

收录：EI(收录号：20133516662835)

语种：英文

外文关键词：Supramolecular chemistry - Cationic surfactants - Block copolymers - Conformations - Hydrophobicity - Aggregates - Complexation

摘要：Decondensation of DNA molecules, previously compacted by cationic gemini surfactant 12-3-12 · 2Br, has been successfully achieved by introducing triblock copolymer (PEO)20-(PPO)70-(PEO)20 (P123). P123 can interact with 12-3-12 · 2Br to form supramolecular assemblies through hydrophobic interactions, while not interacting with DNA. When introducing 12-3-12 · 2Br into P123/DNA system, the presence of P123 will inhibit the formation of DNA/12-3-12 · 2Br complexes due to the stronger interaction between P123 and 12-3-12 · 2Br. For previously formed DNA/12-3-12 · 2Br complexes, the addition of P123 can lead to the release of DNA from the complex, which should be attributed to the complexation of P123 with free 12-3-12 · 2Br surfactants in bulk phase followed by the breakup of the thermodynamic equilibrium between surfactant aggregates associated with DNA and free surfactants in bulk phase. CD experiments reveal that 12-3-12 · 2Br can change the conformation of DNA from typical B-form to ψ-phase by formation of DNA/12-3-12 · 2Br complexes. However, the release of the surfactant from the complex induced by P123 turns DNA conformation from ψ-phase back to B-form. ? 2013 Springer-Verlag Berlin Heidelberg.