文献类型：期刊文献

英文题名：Small molecule microarrays for drug residue detection in foodstuffs

作者：Zuo Peng; Ye Bang-Ce

机构：[1]E China Univ Sci & Technol, Dept Food Engn & Sci, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2006

卷号：54

期号：19

起止页码：6978

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000240465000005)】；

语种：英文

外文关键词：small molecule microarrays; veterinary drug residue; immunoassay

摘要：Microarrays have been used as tools for analyzing biological compositions at different levels. In this study, we proposed a small molecule microarray (SMM) method for detection of three veterinary drug residues, chloramphenicol, clenbuterol, and tylosin, in foodstuffs simultaneously and quantitatively. The small drug molecules were immobilized on the surface of the modified glass slides. Then the mixture of drug corresponding antibodies and standards or samples was added to the reaction area. After incubation, the antigen-antibody binding was detected using cy5 labeled secondary antibody. The calibration curves of the residues were drawn, and they indicated the lowest detection limit the linearity range. The detectable concentrations of the three residues are lower than the maximum residue levels (MRLs). No cross reactivity was found among the three residues. The coefficient of variation of the spot intensities was below 5% in a subarray, and below 15% among subarrays. The spike sample test and the comparison of detection results by SMMs and ELISA demonstrated the accuracy of the proposed SMMs method.