文献类型：期刊文献

英文题名：Micro-electrochemical DO sensor with ultra-micropore matrix fabricated with femtosecond laser processing successfully applied in on-line DO monitoring for yeast culture

作者：Fan, Meng[1];Gu, Zhen[2];Chen, Wei[3];Wang, HuiFeng[2];Zhuang, YingPing[1];Xia, Jianye[1,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Smart Mfg Energy Chem Proc, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Normal Univ, Sch Phys & Elect Sci, XXL Extreme Optoelectromech Lab, Shanghai 200241, Peoples R China;[4]Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China

年份：2023

卷号：45

期号：4

起止页码：449

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20230513462909);WOS:【SCI-EXPANDED(收录号:WOS:000920958600002)】；

基金：This work was financially supported by the National Key Research and Development Program of China (Grant No. 2021YFC2101100).

语种：英文

外文关键词：All-solid electrolyte; Dissolved oxygen sensor; Electrolysis; Real time monitoring; Yeast fermentation

摘要：Accurate monitoring of dissolved oxygen (DO) is vital for aerobic fermentation process control. This work presents an autoclavable Micro-Dissolved oxygen Sensor (MDS) that can monitor real time DO. The proposed sensor is much cheaper to be manufactured (< $35) and can be adapted to varying measurement environments. An ultra-micropore matrix was created using femtosecond laser processing technology to reduce flow dependency of probe signals. The validity of the proposed DO sensor was verified by testing it under different DO levels. The result revealed consistency between the new designed sensor and a commercial DO sensor. The obtained sensitivity was- 7.93 mu A center dot L center dot mg(-1) (MDS with ultra-micropore matrix). Moreover, the MDS can function without an oxygen-permeable membrane and a solid electrolyte was used which reduced the response time (4.6 s). For real-time monitoring, the stability of the MDS was validated during a yeast batch fermentation carried out until 18 h.