文献类型：期刊文献

英文题名：Color development with rational screening method for improved L-glutamate oxidase-producing strains

作者：Li, QS; Wang, LJ; Li, YR

年份：1996

卷号：18

期号：1

起止页码：7

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:A1996TT61400002)】；

语种：英文

外文关键词：L-glutamate; L-glutamate oxidase; screening; mutation

摘要：A rapid and convenient screening method for the detection of a more productive L-glutamate oxidase (GO) producer was developed on the basis of an H2O2-dependent, peroxidase-catalyzed chromogenic reaction method. With this screening method and HNO2 mutagenesis, we obtained a mutant whose productivity of GO in liquid culture was 80 limes that of the parent strain.