文献类型：期刊文献

英文题名：Exploring the Single-Molecule Transient Interactions with Nanopore Frequency Spectrum

作者：Fu, Ying-Huan[1];Li, Xinyi[1];Ma, Linyue[1];Wan, Yong-Jing[2];Zhang, Li-Min[3];Ying, Yi-Lun[1,4];Long, Yi-Tao[1]

机构：[1]Nanjing Univ, Mol Sensing & Imaging Ctr, Sch Chem & Chem Engn, Nanjing 210023, Peoples R China;[2]East China Univ Sci & Technol, Sch Informat Sci & Engn, Shanghai 200237, Peoples R China;[3]Nanjing Univ, Sch Elect Sci & Engn, Nanjing 210023, Peoples R China;[4]Nanjing Univ, Chem & Biomed Innovat Ctr, Nanjing 210023, Peoples R China

年份：2024

卷号：128

期号：3

起止页码：1110

外文期刊名：JOURNAL OF PHYSICAL CHEMISTRY C

收录：;EI(收录号：20240315409843);WOS:【SCI-EXPANDED(收录号:WOS:001151392800001)】；

基金：This research was supported by the National Key R&D Program of China (2022YFA1304604), the National Natural Science Foundation of China (22027806, 22090054, 22090051), and programs for high-level entrepreneurial and innovative talents introduction of Jiangsu Province. The authors especially thank Jun-Ge Li and Huma Bhatti for their kind help in protein engineering and text revision.

语种：英文

外文关键词：Kinetics - Molecules - Nanopores - Spectrum analysis

摘要：Interactions at the single-molecule interface play vital roles in biology and nanotechnology. However, nanopore technology hardly describes the transient interaction kinetics of small molecules due to fast translocation events and weak ionic current fluctuations. Here, we employed an improved frequency spectrum method for analyzing the ion current fluctuations induced by small molecules entering the nanopore. This frequency spectrum analysis method allows for the analysis of transient current changes beyond the instrumental temporal resolution. The parameter (peak amplitude, a(m)) from the frequency spectrum is used for analyzing the transient interaction kinetics on poly-(dA)(2) with the aerolysin nanopore. A nearly consistent linear relationship of a(m) and interaction rate (1/tau(on)) reveals the kinetics of the interaction by the a(m) value. Moreover, we analyzed the ionic current generated by ethylenediaminetetraacetic acid (EDTA) molecules through the aerolysin nanopore. The results demonstrate that kinetics hidden in the open-pore current noise is accessible by this frequency spectrum analysis method, thereby providing insights into elucidating the molecular transient interactions.