文献类型：期刊文献

英文题名：A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila

作者：Zhou, Lingyun[1]; Wang, Xiuhua[2]; Liu, Qin[1]; Wang, Qiyao[1]; Zhao, Yan[1]; Zhang, Yuanxing[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China

年份：2010

卷号：146

期号：1-2

起止页码：25

外文期刊名：Journal of Biotechnology

收录：EI(收录号：20100912734689)

语种：英文

外文关键词：Physiology - Strain - Antigens

摘要：In our previous work, four secretory antigen-delivery systems based on different signal peptides (SPs) (SPhlyA, SPrtxA, SPvah3 and SPempA) have been successfully established in attenuated Vibrio anguillarum. To investigate the potential application of the antigen-delivery systems in V. anguillarum multivalent vector vaccine, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from pathogenic Aeromonas hydrophila, as a putative protective antigen, was fused to the four delivery systems, respectively, and introduced into attenuated V. anguillarum strain MVAV6203 to get four GAPDH-delivery strains in this work. Immunodetection of GAPDH indicated that among the four constructs, the SPempA-mediated GAPDH-delivery strain AV(pGap-empA) showed the highest level of GAPDH expression and secretion, and was determined as the multivalent vaccine candidate. Further immune protection evaluation of AV(pGap-empA) in turbot (Scophtalmus maximus) demonstrated that the vaccine candidate could induce efficient protection against V. anguillarum and A. hydrophila, suggesting that this vector vaccine had great potential in serving as a valuable multivalent live vaccine. ? 2010 Elsevier B.V. All rights reserved.