文献类型：期刊文献

英文题名：Advancing homogeneous networking principles for the development of fatigue-resistant, low-swelling and sprayable hydrogels for sealing wet, dynamic and concealed wounds in vivo

作者：Zhang, Yi[1];Pan, Yanjun[2];Chang, Ronghang[1];Chen, Kangli[1];Wang, Kun[1];Tan, Haoqi[3];Yin, Meng[2];Liu, Changsheng[1];Qu, Xue[1,4,5,6]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Childrens Med Ctr, Sch Med, Dept Pediat Cardiothorac Surg, 1678 Dong Fang Rd, Shanghai 200127, Peoples R China;[3]Shanghai Univ, Suzhou Innovat Ctr, Suzhou 215000, Jiangsu, Peoples R China;[4]Shanghai Univ, Wenzhou Inst, Wenzhou 325000, Peoples R China;[5]Shanghai Frontier Sci Ctr Optogenet Tech Cell Meta, Shanghai 200237, Peoples R China;[6]East China Univ Sci & Technol, 130 Meilong Rd, Shanghai, Peoples R China

年份：2024

卷号：34

起止页码：150

外文期刊名：BIOACTIVE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001214149400001)】；

基金：This research was supported by the National key research and development program (2021YFB3800800) , the National Natural Science Foundation of China (31922041, 11932012, 32171341, 82202334) , the 111 Project (B14018) , Excellence Project of Shanghai Municipal Health Commission (20234Z0003) , the Science and Technology Innovation Project and Excellent Academic Leader Project of Shanghai Science and Technology Committee (21S31901500, 21XD1421100) are acknowledged.

语种：英文

外文关键词：Sprayable hydrogel sealants; Homogeneous network; Fatigue-resistance; Low swelling; Wet dynamic and concealed wound

摘要：Effective sealing of wet, dynamic and concealed wounds remains a formidable challenge in clinical practice. Sprayable hydrogel sealants are promising due to their ability to cover a wide area rapidly, but they face limitations in dynamic and moist environments. To address this issue, we have employed the principle of a homogeneous network to design a sprayable hydrogel sealant with enhanced fatigue resistance and reduced swelling. This network is formed by combining the spherical structure of lysozyme (LZM) with the orthotetrahedral structure of 4-arm-polyethylene glycol (4-arm-PEG). We have achieved exceptional sprayability by controlling the pH of the precursor solution. The homogeneous network, constructed through uniform crosslinking of amino groups in protein and 4-arm-PEG-NHS, provides the hydrogel with outstanding fatigue resistance, low swelling and sustained adhesion. In vitro testing demonstrated that it could endure 2000 cycles of underwater shearing, while in vivo experiments showed adhesion maintenance exceeding 24 h. Furthermore, the hydrogel excelled in sealing leaks and promoting ulcer healing in models including porcine cardiac hemorrhage, lung air leakage and rat oral ulcers, surpassing commonly used clinical materials. Therefore, our research presents an advanced biomaterial strategy with the potential to advance the clinical management of wet, dynamic and concealed wounds.