文献类型：期刊文献

英文题名：Colorimetric and fluorescent detection of GSH with the assistance of CTAB micelles

作者：Song, Lun[1];Tian, Haiyu[1];Pei, Xiaoliang[1];Zhang, Ziyou[1];Zhang, Weibing[1];Qian, Junhong[1,2]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2015

卷号：5

期号：73

起止页码：59056

外文期刊名：RSC ADVANCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000357961800013)】；

基金：This work was financially supported by National 973 Program (No. 2011CB910403), Shanghai Municipal Natural Science Foundation (15ZR1409000) and the open fund of Shanghai Key Laboratory of Chemical Biology (SKLCB-2013-03).

语种：英文

摘要：Two water-soluble colorimetric and turn-on fluorescent probes STP1-2 were rationally designed and synthesized for selective recognition of GSH. Both probes are colorless 4-thioether-1,8-naphthalimide derivatives, which are almost non-fluorescent due to the photoinduced electron transfer from the fluorophore to the 4-nitrobenzene. Upon addition of GSH to STP1/STP2-CTAB solution, obvious spectral responses were observed: the absorption peak shifted from 345 nm to 390 nm companied with similar to 90-fold fluorescence enhancement at 487 nm. A good linear relationship between the fluorescence intensity and GSH concentration was obtained, and the detection limit of GSH was estimated to be 8.40 x 10(-8) mol L-1. The experimental results imply that both probes could be applied in fluorescent imaging of GSH within living cells and in the detection of mercapto-containing proteins as well.