文献类型：期刊文献

英文题名：Synthesis and application of two light-sensitive copolymers forming recyclable aqueous two-phase systems

作者：Chen, Jinpeng[1];Miao, Shu[1];Wan, Junfen[1];Xia, Jinan[2];Cao, Xuejun[1]

机构：[1]E China Univ Sci & Technol, Dept Biochem Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Donghua Univ, Dept Appl Phys, Shanghai 200051, Peoples R China

年份：2010

卷号：45

期号：12

起止页码：1928

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20104613391839);WOS:【SCI-EXPANDED(收录号:WOS:000285325100015)】；

基金：This project was supported by Natural Scientific Foundation of China (20474016), and the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204). Authors appreciate to support from above national funds.

语种：英文

外文关键词：Recycling aqueous two-phase systems; Light-sensitive copolymer; Chlorophyllin sodium copper; Bioseparation engineering; Recovery; Partition coefficients

摘要：Recycling aqueous two-phase systems (ATPS) are hopeful application techniques in bioseparation and biocatalysis engineering areas. In this study, two novel light-sensitive and reversible water-soluble copolymers were synthesized and used in recycling ATPS. Polymer P-NBAC was polymerized by using N-isopropylacrylamide (NIPA), n-butylmethacrylate (BMA), acrylic acid (AA) and chlorophyllin sodium copper salt (CHL) as monomers. Copolymer P-NDBC was synthesized by NIPA, 2-(dimethylamino) ethylmethacrylate (DMAEMA), BMA and CHL as monomers. The synthesis yields of two copolymers are 66.9% and 77.2%, respectively. It can be calculated from H-1 NMR graph that the molar ratios of monomers in P-NBAC (NIPA:BMA:AA:CHL) and P-NDBC (NIPA:DMAEMA:BMA:CHL) was 82:4:1:10 and 16:4:3:1, respectively. They could be precipitated by laser irradiation in 488 nm with the least light density of 1.70 x 10(5) W/m(2). The precipitate energy is 1.79 kJ and 3.52 kJ/g, respectively. Five batches of average recoveries of two copolymers in the ATPS are 96.6% and 97.4%. BSA and L-phenylalanine were partitioned in the novel P-NDBC P-NBAC ATPS, and their partition coefficients were 2.1 and 0.52. By applying this ATPS to partition of penicillin, the best partition coefficient K is 4.25. (C) 2010 Elsevier Ltd. All rights reserved.