文献类型：期刊文献

英文题名：Efficient inhibition of Cronobacter biofilms by chitooligosaccharides of specific molecular weight

作者：Lu, Jun[1,2];Chen, Qiming[1,2];Pan, Bolin[1];Qin, Zhen[1,2];Fan, Liqiang[1,2];Xia, Quanming[1,2];Zhao, Liming[1,2]

机构：[1]East China Univ Sci & Technol, R&D Ctr Separat & Extract Technol Fermentat Ind, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Biobased Mat Engn China Light Ind, Shanghai 200237, Peoples R China

年份：2019

卷号：35

期号：6

外文期刊名：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20192607096144);WOS:【SCI-EXPANDED(收录号:WOS:000469257100008)】；

基金：The work was supported by the China Postdoctoral Science Foundation (2017M611478) and theFundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Cronobacter spp; Biofilms; Chitooligosaccharides

摘要：Biofilms enable Cronobacter spp. to contaminate food, infect infants and resist different environmental stresses, especially desiccation, which is the main reason why Cronobacter can survive in powdered infant formula (PIF) for a long time. Considering the high lethality of Cronobacter infection in infants, it is important to find efficient and safe inhibitors of Cronobacter biofilms. In this study, we found that chitooligosaccharides (COS) with a molecular weight of 2000Da efficiently inhibited Cronobacter biofilms, especially in skim milk broth. The minimum biofilm inhibitory concentration (MBIC77) of COS was as low as 20g/mL, which is lower than that reported in most previous studies. Besides, the elimination rate of COS for Cronobacter mature biofilms was 50% when the concentration was 10mg/mL. COS could significantly inhibit soluble polysaccharide secretion and biofilm cell growth, as well as change the cell membrane permeability of Cronobacter. These might be the possible reasons for COS's efficient inhibition of Cronobacter biofilms. However, during the inhibition, five important genes-related to biofilm formationflhD, flgJ, luxR, ompA, and wcaJwere all up-regulated after COS treatment, except the gene bcsA. In summary, our findings showed that COS could be used as an efficient and safe inhibitor against Cronobacter biofilms for better control of Cronobacter contamination and infection.