文献类型：期刊文献

英文题名：Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite

作者：Sun, Xiaolong[1];Lacina, Karel[1,5];Ramsamy, Elena C.[1];Flower, Stephen E.[1];Fossey, John S.[2];Qian, Xuhong[3];Anslyn, Eric V.[4];Bull, Steven D.[1];James, Tony D.[1]

机构：[1]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England;[2]Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England;[3]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[4]Univ Texas Austin, Dept Chem & Biochem, Austin, TX 78712 USA;[5]Masaryk Univ, CEITEC, Brno 62500, Czech Republic

年份：2015

卷号：6

期号：5

起止页码：2963

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20151700785746);WOS:【SCI-EXPANDED(收录号:WOS:000353223100037)】；

基金：TDJ and XS are grateful for financial support from China Scholarship Council (CSC) and University of Bath Full Fees Scholarship. The Catalysis And Sensing for our Environment (CASE) network is thanked for research exchange opportunities.

语种：英文

外文关键词：Organic acids - Indicators (chemical) - Binding energy - Self assembly - Color - Colorimetry

摘要：Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N, N-dimethylaminomethyl) phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS-NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B-N interaction. Our simple system produces a visible colorimetric change and on-off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).