文献类型：期刊文献

英文题名：Comparative Analysis of Growth, Survival, and Virulence Characteristics of Listeria monocytogenes Isolated from Imported Meat

作者：Pan, Xinye[1];Shen, Jinling[2];Hong, Yi[1];Wu, Yufan[3];Guo, Dehua[2];Zhao, Lina[2];Bu, Xiangfeng[1];Ben, Leijie[1];Wang, Xiang[1]

机构：[1]Univ Shanghai Sci & Technol, Sch Hlth Sci & Engn, Shanghai 200093, Peoples R China;[2]Technol Ctr Anim Plant & Food Inspect & Quarantine, Shanghai 200135, Peoples R China;[3]East China Univ Sci & Technol, Ctr Anal & Test, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2024

卷号：12

期号：2

外文期刊名：MICROORGANISMS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001176530200001)】；

基金：No Statement Available

语种：英文

外文关键词：foodborne pathogen; variability; biological characteristics; virulence; potential pathogenicity

摘要：Listeria monocytogenes is an important foodborne pathogen with worldwide prevalence. Understanding the variability in the potential pathogenicity among strains of different subtypes is crucial for risk assessment. In this study, the growth, survival, and virulence characteristics of 16 L. monocytogenes strains isolated from imported meat in China (2018-2020) were investigated. The maximum specific growth rate (mu(max)) and lag phase (lambda) were evaluated using the time-to-detection (TTD) method and the Baranyi model at different temperatures (25, 30, and 37 degrees C). Survival characteristics were determined by D-values and population reduction after exposure to heat (60, 62.5, and 65 degrees C) and acid (HCl, pH = 2.5, 3.5, and 4.5). The potential virulence was evaluated via adhesion and invasion to Caco-2 cells, motility, and lethality to Galleria mellonella. The potential pathogenicity was compared among strains of different lineages and subtypes. The results indicate that the lineage I strains exhibited a higher growth rate than the lineage II strains at three growth temperatures, particularly serotype 4b within lineage I. At all temperatures tested, serotypes 1/2a and 1/2b consistently demonstrated higher heat resistance than the other subtypes. No significant differences in the log reduction were observed between the lineage I and lineage II strains at pH 2.5, 3.5, and 4.5. However, the serotype 1/2c strains exhibited significantly low acid resistance at pH 2.5. In terms of virulence, the lineage I strains outperformed the lineage II strains. The invasion rate to Caco-2 cells and lethality to G. mellonella exhibited by the serotype 4b strains were higher than those observed in the other serotypes. This study provides meaningful insights into the growth, survival, and virulence of L. monocytogenes, offering valuable information for understanding the correlation between the pathogenicity and subtypes of L. monocytogenes.