文献类型：期刊文献

英文题名：Nitrogen-Boosted H2O2 Production of Arginine-Polyphenol Nanozyme Drives Oxidative Eustress for Hair Regeneration

作者：Wang, Yifei[1];Yang, Yaojia[1];Wang, Aoxue[2,3,4];Shen, Chen[2];Tan, Shenliang[1];Liu, Changsheng[1];Zhao, Yuzheng[2,3,4];Qu, Xue[1,3]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Engn Res Ctr Biomed Mat, Sch Mat Sci & Engn,Minist Educ,Key Lab Ultrafine M, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Shanghai 200237, Peoples R China;[4]Chinese Acad Med Sci, Res Unit New Tech Live Cell Metab Imaging, Beijing 100730, Peoples R China

年份：2026

卷号：13

期号：18

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20254719520059);WOS:【SCI-EXPANDED(收录号:WOS:001614270600001)】；

基金：Y.W. and Y.Y. contributed equally to this work. This work was jointly supported by the National Key Research and Development Program (2024YFA0917700), the National Natural Science Foundation of China (32425031, 32171341 to X.Q, 32530064, 92457301, 32030065, 32121005 to Y.Z.), the Science and Technology Innovation Project of Shanghai Science and Technology Committee (24CL2900800, 25CL2900700), the Zhejiang Natural Science Foundation (Z25E030005). Peak Disciplines (Type IV) of Institutions of Higher Learning in Shanghai (Y.Z.), CAMS Innovation Fund for Medical Sciences (Chinese Academy of Medical Sciences, 2019-I2M-5-013 to Y.Z.), the Shanghai Science and Technology Commission (24HC2810700 to Y.Z.; 25ZR1402109 to A.W.), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：hair regeneration; hydrogen peroxide; nanoenzymes; oxidative eustress; polyphenol

摘要：Reactive oxygen species (ROS)-triggered oxidative eustress can stimulate regenerative signaling, yet its therapeutic window remains narrow. Mitochondrial respiratory complexes and superoxide dismutase (SOD) are canonical enzymatic sources of intracellular H2O2. Here we report a biomimetic polyphenol-amino acid nanozyme (PEAs) that couples the semiquinone radical of coenzyme Q (ubiquinone) with the Arg143 residue of Zn/Cu-SOD1. Through self-assembling epigallocatechin gallate (EGCG) and L-arginine (L-Arg), PEAs enable O2 adsorption and activation with controlled H2O2 generation. The H2O2 output is finely tuned by modulating the nitrogen (N) content from L-Arg. Integrated experimental and computational analyses reveal that the N-sites introduced by L-Arg promote semiquinone electron delocalization, increase semiquinone abundance, thereby strengthening O2 adsorption, facilitating electron/proton transfer, and lowering the reaction barrier for H2O2 synthesis. Using the genetically encoded H2O2 sensor HyPerion, this work validates the sustained intracellular modulation of H2O2 by PEAs. In a mouse model of telogen effluvium, controlled H2O2 delivery activates the follicular niche via Wnt/beta-catenin upregulation and Ca2+/calcineurin/NFAT downregulation, resulting in robust follicle activation and a non-pharmacological approach to alopecia therapy. This polyphenol-amino acid nanozyme therefore provides a safe and effective strategy for in vivo pro-oxidative modulation, offering a tunable H2O2-based platform to harness beneficial oxidative stress for tissue renewal.