文献类型：期刊文献

英文题名：AND-Logic Based Fluorescent Probe for Selective Detection of Lysosomal Bisulfite in Living Cells

作者：Li, Ji-Zhen[1,2];Sun, Yi-Hang[1,2];Wang, Cheng-Yun[1];Guo, Zhi-Qan[1];Shen, Yong-Jia[1,2];Zhu, Wei-Hong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：91

期号：18

起止页码：11946

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20193907466210);WOS:【SCI-EXPANDED(收录号:WOS:000487156900060)】；

基金：This work was supported by the Natural Science Foundation of Shanghai (16ZR1408000) and the National Key Program of China (2016YFA0200302).

语种：英文

外文关键词：Probes - Sulfur dioxide - Biomarkers - Alkalinity - Computer circuits - Biocompatibility - Cytology - Fluorescence

摘要：Sulfur dioxide (SO2) plays significant roles in regulating cell apotosis and inflammation. However, there are complex interactions between small biomolecules in cells, and the identification of these coexisting biomarkers remains a challenge. Herein, we report an AND logic gate based fluorescent probe (NY-Lyso), operating by responding to pH differences between organelles in cell and selectively reacting with bisulfite (HSO3-). This approach allows the fluorescence of the probe to remain silent under neutral or alkaline conditions, notably, is activated by costimulation of lower pH and bisulfite. Furthermore, it was confirmed to be biocompatible and could be employed to monitor HSO3- in lysosomes of living cells. The proposed method demonstrated more practical and outstanding capabilities in targeted and real-time monitoring, providing an effective optical tool for biomarker sensing.