文献类型：期刊文献

英文题名：Efficient reduction of aromatic ketones with NADPH regeneration by using crude enzyme from Rhodotorula cells and mannitol as cosubstrate

作者：Yang, Wei[1]; Xu, Jian-He[1]; Pan, Jiang[1]; Xu, Yi[1]; Wang, Zhi-Long[2]

机构：[1] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China

年份：2008

卷号：42

期号：1

起止页码：1

外文期刊名：Biochemical Engineering Journal

收录：EI(收录号：20083511493814)

语种：英文

外文关键词：Enzymes - Polyols - Aromatic compounds - Drug products

摘要：An enzyme-coupled system, containing a ketoreductase for prochiral ketones reduction and a mannitol dehydrogenase for NADPH regeneration, was discovered from the cell-free extract of Rhodotorula sp. AS2.2241 and used successfully for the asymmetric reduction of 2-acetylpyridine, giving (S)-1-(2-pyridine)ethanol in >99% ee with recycling of NADPH for 4220 times. Moreover, the endogenously coupled enzymes system could also reduce various aromatic ketones such as 3- and 4-acetylpyridines with 96-99% ee. ? 2008.