文献类型：期刊文献

英文题名：Synergy of cystamine and pyraclostrobin against Fusarium graminearum involves membrane permeability mitigation and autophagy enhancement

作者：Luan, Shaorong[1,3];Chen, Yongjun[2];Wang, Xiaohua[1,3];Yan, Dongmei[2];Xu, Jialin[2];Cui, Hairong[1,3];Huang, Qingchun[2]

机构：[1]Wuchang Univ Technol, Coll Life Sci, Wuhan 430223, Peoples R China;[2]Shanghai Key Lab Chem Biol, East China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[3]Wuchang Univ Technol, Coll Life Sci, Synergy Innovat Ctr Biol Peptide Antidiabet Hubei, Wuhan 430223, Peoples R China

年份：2022

卷号：188

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000908320300006)】；

基金：This work is supported in part by the Shanghai Committee of Science and Technology of China (No. 21S11901400, 22N41900100).

语种：英文

外文关键词：Pyraclostrobin; Cystamine; Synergistic action; Membrane permeability; Autophagy; Fusarium graminearum

摘要：The application of fungicide mixture is one of the most important measures to extend the service life of highly selective fungicides. Pyraclostrobin (PYR), which has been extensively used to control plant diseases by inhibiting mitochondrial respiration of pathogenic fungi, is at a high risk of resistance development. In this study, the potential of PYR alone or in combination with cystamine, an inhibitor of microbial transglutaminase, to suppress Fusarium graminearum was tested in vitro and in vivo. A synergistic effect of PYR/CYS mixture was observed both in vitro and when applied to etiolated wheat coleoptile. The control effect of PYR/CYS mixture on F. graminearum was better than that of PYR alone, which was reflected by the increased protection effect. The discrepancies of membrane permeability and the redox-physiological state were observed between PYR and PYR/CYS treatments, suggesting that an increased PYR availability in F. graminearum mycelia could be related with the observed synergistic action. Moreover, a synergistic profile was observed between PYR and CYS in regard of massive autophagosomes in mycelia, indicating that enhanced autophagy could be involved in the mode of action of PYR/CYS mixture. The differential content of mitochondrial metabolites between PYR and PYR/CYS treatments also provided evidence for CYS contribution to the fungicidal action of PYR/CYS mixture. The results provide insight into the synergistic mechanism of action of PYR/CYS mixture and an effective way to enhance the efficiency of PYR to combat F. graminearum.