文献类型：期刊文献

英文题名：A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate

作者：Hang, Haifeng[1]; Ye, Xinhao[1]; Guo, Meijin[1,2]; Chu, Ju[1]; Zhuang, Yingping[1]; Zhang, Ming[1]; Zhang, Siliang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] Department of Biotechnology, Jiangxi Agricultural University, 10 Yingshang Road, Nanchang, 330045, China

年份：2009

卷号：44

期号：4

起止页码：185

外文期刊名：Enzyme and Microbial Technology

收录：EI(收录号：20090611895433)

语种：英文

外文关键词：Substrates - Yeast - Fermentation - Methanol - Recombinant proteins - Glucose oxidase - Glucose sensors

摘要：The methylotrophic yeast Pichia pastoris has become a popular host for the production of recombinant protein under the tight control of methanol-induced alcohol oxidase promoter (PAOX1), which is, however, strongly repressed by glucose at the transcription level. A real-time parameter-based controlled glucose feed strategy (rpcGFS) is developed here to realize Pichia high-cell density culture. It allows for the high-level production of recombinant phytase by P. pastoris YY113 using glucose as the growth substrate instead of glycerol. As a result, high phytase production (2200 FTU phytase ml-1) and yield (0.25 FTU ml-1[phytase] g-1[methanol]) are achieved successfully, respectively, after 100 h methanol induction by this novel simple fermentation strategy. ? 2008 Elsevier Inc. All rights reserved.