文献类型：期刊文献

中文题名：有机溶剂中脂肪酶催化的2-辛醇动力学拆分

英文题名：Kinetic Resolution of 2 Octanol Catalyzed by Lipase in Organic Solvent

作者：朱洁[1];刘洪湖[1];胡英[1];许建和[2];曹淑桂[3]

机构：[1]华东理工大学化学系;[2]华东理工大学生物反应器工程国家重点实验室;[3]吉林大学酶工程实验室

年份：1998

卷号：12

期号：5

起止页码：323

中文期刊名：分子催化

外文期刊名：Journal of Molecular Catalysis(China)

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金

语种：中文

中文关键词：辛醇;动力学;拆分;脂肪酶;对映选择性;酯化反应

外文关键词：Octanol, Kinetic resolution, Lipase, Enantioselective esterification, Organic solvent system

摘要：利用有机溶剂中假单胞菌脂肪酶催化的对映选择性酯化反应，对外消旋（Ｒ，Ｓ）－２－辛醇进行了光学拆分；分别考察了冻干时酶缓冲液的ｐＨ值和浓度以及２－辛醇酯化的酰基给体（脂肪酸）、有机溶剂、反应温度等因素对脂肪酶催化性能的影响．结果表明，冻干时酶缓冲液的ｐＨ值和浓度对酶的活力影响很大，而对选择性影响较小．以ｐＨ值为８．３、浓度为２０ｍｍｏｌ／Ｌ的磷酸盐缓冲液最为合适．不同链长的脂肪酸对酶活性和选择性的影响均十分显著，以中等链长的癸酸作为酰基给体时效果最好．在亲水性很强（ｌｏｇＰ＜１）的极性溶剂中，酶几乎没有活力；在较强疏水性（２＜ｌｏｇＰ＜３）的溶剂中，酶具有中等活力，但在甲苯（ｌｏｇＰ＝２．５）中酶的对映选择性最好；在强疏水性（ｌｏｇＰ＞３）溶剂中，酶的活力较高，尤以正己烷中的活力为最高．酶反应的最适温度在４５℃左右．温度较高时，反应的选择性显著提高，但酶的稳定性急剧下降．
Racemic 2 octanol was kinetically resolved through enantioselective esterification catalyzed by Pseudomonas sp. lipase in organic solvent systems. Factors affecting catalytic performance of the lipase were investigated respectively. Both pH value and concentration of the phosphate buffer used for lyophilization of the enzyme have profound effects on enzyme activity but have little effects on enzyme selectivity, with an optimum at pH value 8.3 and 20 mmol/L. The effects of various fatty acids on either activity or enantioselectivity of the enzyme are significant and best results are obtained using decanoic acid as the acyl donor. When the lipase reaction is carried out in a strong polar hydrophilic solvent(log P <1) almost no activity is revealed. A moderate activity is observed in a relatively hydrophobic solvent (23), with the highest in n hexane. The optimum temperature of enzyme reaction is around 45℃, above which the enantioselectivity is considerably enhanced but the enzyme activity decreases rapidly.