文献类型：期刊文献

英文题名：The impact of Paenibacillus polymyxa HY96-2 luxS on biofilm formation and control of tomato bacterial wilt

作者：Yi, Jincui[1];Zhang, Daojing[1];Cheng, Yuejuan[1];Tan, Jingjing[1];Luo, Yuanchan[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：103

期号：23-24

起止页码：9643

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20194607677311);WOS:【SCI-EXPANDED(收录号:WOS:000493964800002)】；

基金：This work was financially supported by the National Key Research and Development Program of China (2017YFD0201107-2-3) and the National Natural Science Fund (31501693).

语种：英文

外文关键词：Paenibacillus polymyxa; Quorum sensing; luxS; Biofilm formation; Bacterial wilt; Biocontrol efficacy

摘要：The focus of this study was to investigate the effects of luxS, a key regulatory gene of the autoinducer-2 (AI-2) quorum sensing (QS) system, on the biofilm formation and biocontrol efficacy against Ralstonia solanacearum by Paenibacillus polymyxa HY96-2. luxS mutants were constructed and assayed for biofilm formation of the wild-type (WT) strain and luxS mutants of P. polymyxa HY96-2 in vitro and in vivo. The results showed that luxS positively regulated the biofilm formation of HY96-2. Greenhouse experiments of tomato bacterial wilt found that from the early stage to late stage postinoculation, the biocontrol efficacy of the luxS deletion strain was the lowest with 50.70 +/- 1.39% in the late stage. However, the luxS overexpression strain had the highest biocontrol efficacy with 75.66 +/- 1.94% in the late stage. The complementation of luxS could restore the biocontrol efficacy of the luxS deletion strain with 69.84 +/- 1.09% in the late stage, which was higher than that of the WT strain with 65.94 +/- 2.73%. Therefore, we deduced that luxS could promote the biofilm formation of P. polymyxa HY96-2 and further promoted its biocontrol efficacy against R. solanacearum.