文献类型：期刊文献

英文题名：Subtyping Novel Zoonotic Pathogen Cryptosporidium Chipmunk Genotype I

作者：Guo, Yaqiong[1,2];Cebelinski, Elizabeth[3];Matusevich, Christine[4];Alderisio, Kerri A.[5];Lebbad, Marianne[6];McEvoy, John[7];Roellig, Dawn M.[2];Yang, Chunfu[8];Feng, Yaoyu[1];Xiao, Lihua[2]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA USA;[3]Minnesota Dept Hlth, Publ Hlth Lab, St Paul, MN USA;[4]Vermont Dept Hlth Lab, Burlington, VT USA;[5]New York City Dept Environm Protect, Valhalla, NY USA;[6]Publ Hlth Agcy Sweden, Solna, Sweden;[7]N Dakota State Univ, Dept Vet & Microbiol Sci, Fargo, ND 58105 USA;[8]Ctr Dis Control & Prevent, Div Global HIV AIDS, Ctr Global Hlth, Atlanta, GA USA

年份：2015

卷号：53

期号：5

起止页码：1648

外文期刊名：JOURNAL OF CLINICAL MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000353036500025)】；

基金：This work was supported by the National Natural Science Foundation of China (31229005, 31110103901, and 31425025), the Water Special Project (2014ZX07104006), the National Special Fund for State Key Laboratory of Bioreactor Engineering (no. 2060204), and the U.S. Centers for Disease Control and Prevention.

语种：英文

摘要：Cryptosporidium chipmunk genotype I is an emerging zoonotic pathogen in humans. The lack of subtyping tools makes it impossible to determine the role of zoonotic transmission in epidemiology. To identify potential subtyping markers, we sequenced the genome of a human chipmunk genotype I isolate. Altogether, 9,509,783 bp of assembled sequences in 853 contigs were obtained, with an N50 of 117,886 bp and >200-fold coverage. Based on the whole-genome sequence data, two genetic markers encoding the 60-kDa glycoprotein (gp60) and a mucin protein (ortholog of cgd1_470) were selected for the development of a subtyping tool. The tool was used for characterizing chipmunk genotype I in 25 human specimens from four U.S. states and Sweden, one specimen each from an eastern gray squirrel, a chipmunk, and a deer mouse, and 4 water samples from New York. At the gp60 locus, although different subtypes were seen among the animals, water, and humans, the 15 subtypes identified differed mostly in the numbers of trinucleotide repeats (TCA, TCG, or TCT) in the serine repeat region, with only two single nucleotide polymorphisms in the nonrepeat region. Some geographic differences were found in the subtype distribution of chipmunk genotype I from humans. In contrast, only two subtypes were found at the mucin locus, which differed from each other in the numbers of a 30-bp minisatellite repeat. Thus, Cryptosporidium chipmunk genotype I isolates from humans and wildlife are genetically similar, and zoonotic transmission might play a potential role in human infections.