文献类型：期刊文献

英文题名：Enhanced acetoin production by serratia marcescens H32 using statistical optimization and a two-stage agitation speed control strategy

作者：Sun, Jianan[1]; Zhang, Liaoyuan[2]; Rao, Ben[1]; Han, Yunbin[1]; Chu, Ju[1]; Zhu, Jiawen[3]; Shen, Yaling[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200-237, China; [2] School of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350-002, China; [3] Chemical Engineering Research Center, East China University of Science and Technology, Shanghai 200-237, China

年份：2012

卷号：17

期号：3

起止页码：598

外文期刊名：Biotechnology and Bioprocess Engineering

收录：EI(收录号：20123315333480)

语种：英文

外文关键词：Bottles - Speed - Speed control - Productivity

摘要：Enhanced acetoin production was carried out by Serratia marcescens H32. First, medium compositions were optimized statistically for shake flask fermentations to produce acetoin. Sucrose and corn steep liquor powder (CSLP) were identified as the most significant factors by Plackett-Burman design. The path of steepest ascent and response surface methodology were then employed to determine the optimal concentrations of the two factors. Acetoin yield was up to 41.5 g/L in flask fermentations using the optimized medium. Furthermore, the optimal medium was used to conduct fermentation experiments in a 3.7-L bioreactor. The influences of different agitation speeds on acetoin production were investigated. Based on a process analysis, a two-stage agitation speed control strategy was proposed, in which the agitation speed was controlled at 700 rpm during the first 8 h and then switched to 600 rpm. A relatively high acetoin concentration (44.9 g/L) and high acetoin productivity (1.73 g/L/h) were achieved by applying this strategy. Fed-batch fermentation based on the two-stage agitation speed control strategy was performed, and a maximum acetoin concentration of 60.5 g/L with productivity of 1.44 g/L/h was achieved. ? 2012 The Korean Society for Biotechnology and Bioengineering and Springer.