文献类型：期刊文献

英文题名：Tris(1,3-dichloro-2-propyl) phosphate accelerated the aging process induced by the 4-hydroxynon-2-enal response to reactive oxidative species in Caenorhabditis elegans

作者：Wang, Chen[1];An, Jing[2];Bai, Yingchen[3];Li, Hui[1,2];Chen, Haibo[4];Ou, Dong[1];Liu, Yongdi[1]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Shanghai Univ, Sch Environm & Chem Engn, Inst Environm Pollut & Hlth, Shanghai 200444, Peoples R China;[3]Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 10012, Peoples R China;[4]South China Inst Environm Sci, MEP, Ctr Environm Hlth Res, Guangzhou 510535, Guangdong, Peoples R China

年份：2019

卷号：246

起止页码：904

外文期刊名：ENVIRONMENTAL POLLUTION

收录：;EI(收录号：20190406422555);WOS:【SCI-EXPANDED(收录号:WOS:000458222100103)】；

基金：This work was supported jointly by the National key research and development plan (2016YFCO206200), the National Natural Science Foundation of China (41877377), Program of Shanghai Academic/Technology Research Leader (18XD1424100), Open Foundation of State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental Sciences (SKLECRA2016OFP19), the Fok Ying Tung Education Foundation (141077), and the Innovation Program of the Shanghai Municipal Education Commission (14ZZ059). We also would like to thank the anonymous referees for their helpful comments on this paper.

语种：英文

外文关键词：Tris(1,3-dichloro-2-propyl) phosphate; Aging; ROS; 4-Hydroxynon-2-enal; GST

摘要：Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) has been frequently detected in environmental media and biological samples. However, knowledge of its adverse health consequences is limited. In the current study, Caenorhabditis elegans (C. elegans, L1 larvae) were exposed to TDCPP at environmentally relevant concentrations (control, 0.1, 1, 100 and 1000 mu g L-1) for 72 h to explore any association between TDCPP and the aging process. Some of the degenerative age-related indicators were observed, including locomotion behaviors and lifespan. As crucial biomarkers of aging, the accumulation of lipofuscin, and lipid peroxidation (LPO) products exemplified by 4-hydroxynon-2-enal (4-HNE) were detected. This product forms as a result of oxidative stress, as confirmed by an N-acetyl-L-cysteine (NAC) pharmacological assay. Moreover, a significant increase in reactive oxide species (ROS) production in a dose-dependent manner using a fluorescent probe was observed. For the underlying molecular mechanism of the above aging phenotypes, significantly upregulated transcription of genes related to antioxidant systems, especially a subset of glutathione S-transferase (gst-5, gst-6, gst-9, gst-10, gst-19, gst-24, gst-26, gst-29, gst-33, and gst-38), was found by RNA-Seq and further confirmed by RT-qPCR. The elevated glutathione S-transferase (GST) was attributed to the significant increase in 4-HNE because mutations in gst-5 and gst-24 inhibited the conjugation of GSTs with 4-HNE. Therefore, GST play an indispensable role in the detoxification process of TDCPP exposure and further confirmed LPO accumulation at the molecular mechanism level. In conclusion, TDCPP accelerated the aging process induced by the LPO products, 4-HNE, response to reactive oxidative species in C. elegans. (C) 2019 Elsevier Ltd. All rights reserved.