文献类型：期刊文献

英文题名：Development of an economical fermentation platform for enhanced ansamitocin P-3 production in Actinosynnema pretiosum

作者：Liu, Ting[1];Bessembayeya, Lyailya[1];Chen, Jun[1];Wei, Liu-Jing[1];Hua, Qiang[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：6

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513078365);WOS:【SCI-EXPANDED(收录号:WOS:000461498800001)】；

基金：This study was partly financially supported by National Natural Science Foundation of China (21776081, 21576089).

语种：英文

外文关键词：Ansamitocin P-3; Cane molasses; Soybean powder; Medium optimization

摘要：Background: Ansamitocin P-3 (AP-3), produced by Actinosynnema pretiosum, has extraordinary antitumor activity and has been used as toxic "warhead" in antibody-conjugated drugs. However, AP-3 production is limited by its low yield and thus the high cost of fermentation. In this study, we aimed to reduce fermentation costs by utilizing the low-cost substrates and improve the AP-3 production, simultaneously. Result: In this study, low-cost substrates used for ansamitocins production were selected via single-factor experiments and then the concentrations of these suitable carbon and nitrogen sources were optimized by response surface method (RSM). Cane molasses, glycerol, and cold-pressed soybean powder were determined to be the most suitable economical carbon and nitrogen sources for AP-3 accumulation. The AP-3 titer in shaking flasks reached 111.9 mg/L using the optimized medium containing cane molasses (63.22 g/L), glycerol (22.91 g/L), and cold-pressed soybean powder (3.29 g/L). In addition, appropriate supplementation with isobutanol, soybean oil, and vitamin B-1 resulted in a marked enhancement in AP-3 production, to 141 mg/L. Meanwhile, the cost of this optimized fermentation medium was around 50% lower than those of two other high-yielding media. Conclusion: Our findings provided practical and economically competitive cultivation conditions for the production of a value-added antitumor agent and may thus extend the applications of ansamitocin P-3.