文献类型：期刊文献

英文题名：Glutamate-related nitrogen metabolism regulates cold-adaptive synthesis of red pigment in polar fungus Geomyces sp. WNF-15A

作者：Zhou, Jiawei[1];Long, Haoyu[1];Guo, Yan[1];Lu, Jian[1];Wang, Nengfei[2];Liu, Haifeng[3];Zhou, Xiangshan[4];Cai, Menghao[1,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China;[3]China Resources Angde Biotech Pharm Co Ltd, 78 Ejiao St, Liaocheng, Peoples R China;[4]China Resources Biopharmaceut Co Ltd, Sightseeing Rd, Shenzhen 130184, Peoples R China;[5]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2025

卷号：404

起止页码：121

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20251718288339);WOS:【SCI-EXPANDED(收录号:WOS:001480246500001)】；

基金：This study was supported by the National Natural Science Foundation of China (42176238) and the 111 Project of China (B18022) . We thank Shanghai Sangon Biotech Co., Ltd. for the support of tran-scriptome sequencing.

语种：英文

外文关键词：Cold adaptation; Glutamate; Nitrogen metabolism; Polar fungi; Red pigments

摘要：The polar fungus Geomyces sp. WNF-15A produces high-quality red pigment (AGRP), but the cold-dependent characteristic of AGRP synthesis restricts its industrialization. This study employed transcriptome analysis to compare gene expression profiles of the wild-type strain with cold-independent mutants of scaffold1.t692 (Delta 1-692) and scaffold2.t704 (Delta 2-704). From the analysis, 23 candidate genes were identified and functionally characterized among 22,600 differentially expressed genes. Knockout and recovery of scaffold5.t61, scaffold7. t586, or scaffold7.t712 proved their regulatory functions in AGRP synthesis, among which scaffold5.t61 functioned as a transcription factor, while scaffold7.t586 and scaffold7.t712 were involved in the glutamate-related nitrogen metabolism. Exogenous addition of nitrate, glutamine, and glutamate, combined with transcriptional regulation studies, revealed the importance of glutamate metabolism for cold-adaptive synthesis of AGRP. Scaffold5.t61 responded to the cold environment and regulated the transcription of scaffold2.t704 and scaffold1. t692. It subsequently increased glutamate synthesis by regulating the key nitrogen metabolism genes of scaffold7. t586 and scaffold7.t712, ultimately resulting in cold-dependent synthesis of AGRP in Geomyces sp. WNF-15A. This study offers new insights into the mechanisms of cold adaptation in polar fungi and serves as a reference for the development of psychrophilic fungal resources.