文献类型：期刊文献

中文题名：基于核酸适体微球的沙门氏菌检测方法研究

英文题名：Study on detection method of salmonella using aptamer- conjugated microbeads

作者：金娜[1];孔先利[2];林敏[1];左鹏[3]

机构：[1]温州出入境检验检疫局,浙江温州325000;[2]台州出入境检验检疫局,浙江台州318000;[3]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2015

卷号：36

期号：13

起止页码：321

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

语种：中文

中文关键词：沙门氏菌;适体;微球;检测

外文关键词：salmonella;aptamer; microbeads ; detection

摘要：目的:研究基于核酸适体的微球芯片系统应用于沙门氏菌检测的可行性。方法:采用偶联有沙门氏菌适体的微球作为载体,以荧光作为检测信号建立微球芯片体系,并优化影响检测结果的各项因素。结果:通过此方法检测20份水产样品,6份检出沙门氏菌,检出率为30%。结论:建立的检测体系能实现对沙门氏菌的快速,准确的检测,且具有较高的特异性。
Objective. To investigate the feasibility of aptamer- conjugated microbeads for the detection of salmonella.Methods.Amino-terminal aptamer probes were covalently attached onto the surface of microbeads. Fluorescence from microbeads was detected as signal source, and parameters were optimized which may affect detection of the target.Results=Salmonella were detected in six of 20 aquatic product samples by this method,and the detection rate was 30%.Conclusion：The aptamer-conjugated microbeads can be used for high throughput and accurate detection of salmonella.