文献类型：期刊文献

中文题名：基于表面增强拉曼光谱的酪氨酸酶可抛式传感器研究

英文题名：Disposable Surface-enhanced Raman Scattering Sensor for Detection of Tyrosinase Activity

作者：甘振飞[1];汪璐[1];郭丹[1];李大伟[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2019

卷号：47

期号：4

起止页码：613

中文期刊名：分析化学

外文期刊名：Chinese Journal of Analytical Chemistry

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000470963000019)】；北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 21777041, 21575041) and the Shanghai Pujiang Program, China (No. 17PJD010).

语种：中文

中文关键词：表面增强拉曼光谱;酪氨酸酶;传感器;可抛式

外文关键词：Surface-enhanced Raman scattering;Tyrosinase;Sensor;Disposable

摘要：研制了一种可抛式表面增强拉曼光谱(SERS)传感器用于检测血清中酪氨酸酶(TYR)活性。首先制备了功能化银纳米颗粒浆料,采用丝网印刷技术制备SERS传感器。修饰在传感器上的4-巯基邻苯二酚可被酪氨酸酶催化氧化为4-巯基邻苯二醌,引起传感器SERS光谱的显著变化,从而实现对酪氨酸酶的检测。此传感器对酪氨酸酶响应快速,在优化条件下5 min内即可完成检测;特征SERS信号强度与酪氨酸酶浓度在1～100 U/mL范围内呈良好的线性关系,检出限为0.28 U/mL(3σ)。由于检测反应的特异性和SERS技术可提供指纹信息的综合优势,此传感器有较好的抗干扰性能。血清样品中的加标回收率为88.1%～104.6%,相对标准偏差(RSD)小于4%,可准确反映苯甲酸的含量及其对酪氨酸酶的抑制能力。结果表明,所研制的可抛式SERS传感器可一次性使用,适合生物样品中酪氨酸酶的快速分析,以及酪氨酸酶抑制剂的快速筛选和检测。
A novel disposable surface-enhanced Raman scattering(SERS) sensor based on enzyme-catalyzed oxidation was developed for detecting tyrosinase(TYR) activity. The SERS sensor was fabricated by assembling the synthesized 4-thiol-catechol onto the surface of silver nanoparticles(AgNPs) which were screen printed on supporting material, and then the detection of TYR activity was accomplished according to SERS spectral changes of the SERS senor caused by the reaction between 4-thiol-catechol and TYR. The results showed that the SERS sensor had fast response to TYR(less than 5 min), and there was a linear relationship between the characteristic SERS signal and TYR concentration from 1 U/mL to 100 U/mL with limit of detection(LOD) of 0.28 U/mL(3σ). Moreover, resulting from the fingerprinting advantage of SERS technique and the specific property of the employed sensing-reaction, the developed SERS sensor had good selectivity towards TYR, which could be used for detection and evaluation of TYR and TYR inhibitor in serum.