文献类型：期刊文献

英文题名：Facile synthesis of layered mesoporous covalent organic polymers for highly selective enrichment of N-glycopeptides

作者：Ding, Fengjuan[1];Chu, Zhanying[1];Zhang, Quanqing[2,3];Liu, Haiyan[1];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China;[3]Fudan Univ, State Key Lab Mol Engn, Shanghai 200433, Peoples R China

年份：2019

卷号：1057

起止页码：145

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20190806523832);WOS:【SCI-EXPANDED(收录号:WOS:000459978400016)】；

基金：This research was financially supported by the National Natural Science Foundation of China (No. 21475044, 51472121), the National Key Technology R&D Program (2015BAK44B00), the National Key Scientific Instrument and Equipment Development Project (No. 2012YQ004403), and the Fundamental Research Funds for the Central Universities (222201817022).

语种：英文

外文关键词：Hydrophilic interaction chromatography; Glycopeptide enrichment; Solvothermal interaction; Mesoporous covalent organic polymer

摘要：Hydrophilic interaction liquid chromatography is a significant strategy for the separation and enrichment of glycoproteins and glycopeptides. A layered imine-based covalent organic polymer with mesopores (denoted as p-TpBDH) was successfully fabricated by a facile solvothermal method. Then p-TpBDH-OH with abundant hydrophilic groups was evolved from p-TpBDH by the direct reduction. 36 and 40 glycopeptides were identified from IgG digests respectively by p-TpBDH and p-TpBDH-OH. Furthermore, the p-TpBDH-OH exhibits superior selectivity (IgG: BSA = 1: 250) for glycopeptides compared with the p-TpBDH. Encouragingly, a total of 463 glycopeptides assigned to 173 glycoproteins were finally identified from only 2 mu L human serum by the p-TpBDH-OH. Compared with p-TpBDH, abundant hydrophilic and nitrogen-containing affinity sites of p-TpBDH-OH facilitate effective hydrophilic interaction between the polymeric material and glycopeptides. All the results demonstrate the functionalized hydrophilic covalent organic polymer has great potential in large-scale N-glycoproteomic research. (C) 2019 Elsevier B.V. All rights reserved.