文献类型：期刊文献

中文题名：Effect of temperature and surfactant on biomass growth and higher?alcohol production during syngas fermentation by Clostridium carboxidivorans P7

作者：Shaohuang Shen[1];Guan Wang[1];Ming Zhang[1];Yin Tang[1];Yang Gu[2];Weihong Jiang[2];Yonghong Wang[1];Yingping Zhuang[1]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;[2]Key Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China.

年份：2020

卷号：7

期号：1

起止页码：120

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：supported by the National Natural Science Foundation of China(31630003);Science and Technology Commission of Shanghai Municipality(17JC1404800);the Youth Innovation Promotion Association of the Chinese Academy of Sciences(membership 2012213).

语种：英文

中文关键词：Clostridium carboxidivorans P7;Higher alcohol;Two-step temperature culture;Surfactant;Comparative transcriptome

摘要：Hexanol–butanol–ethanol fermentation from syngas by Clostridium carboxidivorans P7 is a promising route for biofuel production.However,bacterial agglomeration in the culture of 37°C severely hampers the accumulation of biomass and products.To investigate the effect of culture temperature on biomass growth and higher-alcohol production,C.carboxidivorans P7 was cultivated at both constant and two-step temperatures in the range from 25 to 37°C.Meanwhile,Tween-80 and saponin were screened out from eight surfactants to alleviate agglomeration at 37°C.The results showed that enhanced higher-alcohol production was contributed mainly by the application of two-step temperature culture rather than the addition of anti-agglomeration surfactants.Furthermore,comparative transcriptome revealed that although 37°C promoted high expression of genes involved in the Wood–Ljungdahl pathway,genes encoding enzymes catalyzing acyl-condensation reactions associated with higher-alcohol production were highly expressed at 25°C.This study gained greater insight into temperature-effect mechanism on syngas fermentation by C.carboxidivorans P7.