文献类型：期刊文献

英文题名：High efficiency cell-recycle continuous sodium gluconate production by Aspergillus niger using on-line physiological parameters association analysis to regulate feed rate rationally

作者：Lu, Fei[1];Li, Chao[1];Wang, Zejian[1];Zhao, Wei[2];Chu, Ju[1];Zhuang, Yingping[1];Zhang, Siliang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shan Dong Fuyang Biol Technol Co Ltd, Dezhou, Shandong, Peoples R China

年份：2016

卷号：220

起止页码：433

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20163702796605);WOS:【SCI-EXPANDED(收录号:WOS:000384712900054)】；

基金：This work was financially supported by grants from National High Technology Research and Development Program (2015AA021005), Major State Basic Research Development Program of China (973 Program) (No. 2013CB733600 and 2012CB72100x) and NWO-MoST Joint Program (2013DFG32630). We also thank Shan Dong Fuyang Biological Technology Co., Ltd (DeZhou, China) for donating the industrial strain of A. niger.

语种：英文

外文关键词：Continuous fermentation; Sodium gluconate; Aspergillus niger; Feedback control; On-line parameters

摘要：In this paper, a system of cell-recycle continuous fermentation for sodium gluconate (SG) production by Aspergillus niger (A. niger) was established. Based on initial continuous fermentation result (100.0 h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5 h). Due to less auxiliary time, highest SG production rate (31.05 +/- 0.29 g L-1 h(-1)) and highest yield (0.984 +/- 0.067 mol mol(-1)), overall SG production capacity (975.8 +/- 5.8 g h(-1)) in 50-L fermentor of improved continuous fermentation increased more than 300.0% compared to that of batch fermentation. Improvement of mass transfer and dispersed mycelia morphology were the two major reasons responsible for the high SG production rate. This system had been successfully applied to industrial fermentation and SG production was greatly improved. (C) 2016 Elsevier Ltd. All rights reserved.