文献类型：期刊文献

中文题名：Assembly of a highly stable luminescent Zn5 cluster and application to bio-imaging

英文题名：Assembly of a highly stable luminescent Zn5 cluster and application to bio-imaging

作者：Ze-hui LIU[1];Jin HUANG[1];Ming-hua ZENG[2];Zheng YIN[2]

机构：[1]Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology;[2]Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources,Guangxi Normal University

年份：2017

卷号：31

期号：10

起止页码：1022

中文期刊名：中国药理学与毒理学杂志

外文期刊名：Chinese Journal of Pharmacology and Toxicology

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：supported by Distinguished Young Scholars(21525101);the NSFC(91422302,and 21371037)

语种：英文

中文关键词：3d metal coordination cluster;bio imaging fluorescence probes;pH sensitivity;Zn clusters

外文关键词：3d metal coordination cluster bio imaging fluorescence probes pH sensitivity Zn clusters

摘要：OBJECTIVE To explore a novel pH-sensitive fluorescent probe for in vivo tumor imaging.METHODS Zn5 were obtained in 140℃ after mixed with Me OH,water,Zn(NO_3)2·6 H_2O,H4L and trimethylamine.The fluorescence spectra of Zn5 with the same concentration in different pH aqueous solutions were detected.And the stability of Zn5 was investigated by time dependent fluorescence emission spectra of Zn5 in BSA aqueous solution and 5.0% serum solution.Then,the cytotoxicity of Zn5 was detected by MTT assays.To clarify whether a similar fluorescence response occurs in biological organisms,He La cells were pretreated with probe Zn5(0.5 μmol·L^(-1)) and fluorescence imaging were collected for targeting lysosomes in living cells because of lysosomes′ acidic microenvironment.The A375 tumor-bearing mice were used to assess the imaging ability of Zn5 in vivo.Mouse tumor xenografts were established by injection of A375 cells with 2×10~6 cells per flank.Probe(1 μg·g^(-1)) was administered to mice by injection.Images were obtained using IVIS Spectrum CT Imaging System.RESULTS There is a 11-fold intensity increasing as the pH values changing from 8 to 2.The almost unchanged emission intensities suggest Zn5 is stable in both BSA and serum.Zn5 has negligible cytotoxicity for He La,293 T and CHO-K1 cells.Zn5 can selectively display lysosomes in living cells.Both the 2D and 3D images in vivo distinguish the tumor from other tissues with good fluorescence contrast.CONCLUSION The high chemical stability,emission in the Vis/NIR range,pH sensitivity,a pKa located in the tumor pH range,and low toxicity make Zn5 is suitable for application as a pH-sensitive fluorescent probe for bio-imaging.
OBJECTIVE To explore a novel pH-sensitive fluorescent probe for in vivo tumor imaging.METHODS Zn5 were obtained in 140℃ after mixed with Me OH,water,Zn（NO_3）2·6 H_2O,H4L and trimethylamine.The fluorescence spectra of Zn5 with the same concentration in different pH aqueous solutions were detected.And the stability of Zn5 was investigated by time dependent fluorescence emission spectra of Zn5 in BSA aqueous solution and 5.0% serum solution.Then,the cytotoxicity of Zn5 was detected by MTT assays.To clarify whether a similar fluorescence response occurs in biological organisms,He La cells were pretreated with probe Zn5（0.5 μmol·L^（-1）） and fluorescence imaging were collected for targeting lysosomes in living cells because of lysosomes′ acidic microenvironment.The A375 tumor-bearing mice were used to assess the imaging ability of Zn5 in vivo.Mouse tumor xenografts were established by injection of A375 cells with 2×10~6 cells per flank.Probe（1 μg·g^（-1）） was administered to mice by injection.Images were obtained using IVIS Spectrum CT Imaging System.RESULTS There is a 11-fold intensity increasing as the pH values changing from 8 to 2.The almost unchanged emission intensities suggest Zn5 is stable in both BSA and serum.Zn5 has negligible cytotoxicity for He La,293 T and CHO-K1 cells.Zn5 can selectively display lysosomes in living cells.Both the 2D and 3D images in vivo distinguish the tumor from other tissues with good fluorescence contrast.CONCLUSION The high chemical stability,emission in the Vis/NIR range,pH sensitivity,a pKa located in the tumor pH range,and low toxicity make Zn5 is suitable for application as a pH-sensitive fluorescent probe for bio-imaging.