文献类型：期刊文献

英文题名：PCR-RFLP to Detect Codon 248 Mutation in Exon 7 of p53 Tumor Suppressor Gene

作者：Ouyang, Liming[1];Ge, Chongtao[1];Wu, Haizhen[1];Li, Suxia[1];Zhang, Huizhan[1]

机构：[1]E China Univ Sci & Technol, Sch Biotechnol, Dept Appl Biol, Shanghai 200237, Peoples R China

年份：2009

卷号：37

期号：2

起止页码：106

外文期刊名：BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000264643500007)】；

基金：This work is supported by Innovation Education Foundation Grant of East China University of Science and Technology.

语种：英文

外文关键词：PCR-RFLP; p53 gene; codon 248; mutation; genomics experiment

摘要：Individual genome DNA was extracted fast from oral swab and followed up with PCR specific for codon 248 of p53 tumor suppressor gene. MspI restriction mapping showed the G-C mutation in codon 248, which closely relates to cancer susceptibility. Students learn the concepts, detection techniques, and research significance of point mutations or single nucleotide polymorphisms in individual genomes.